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The infectivity of obtained clones was verified by reinoculation to Nicotiana tabacum cv.
Integrity of the clones was verified by sequencing.
Integrity of the clones was verified by sequencing and protein expression.
The subtracted library was sequenced and the differential expression of selected clones was verified by qPCR.
Sequence of TUB2, tub2-S172A tub2-S172E172E in these clones was verified by PCR as above.
The authenticity of these clones was verified by sequencing, and designated c.2865T and c.2865C, respectively.
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Clones were verified by DNA sequencing prior to expression studies.
Positive clones were verified by DNA sequence analysis.
The PCR clones were verified by sequence alignment with the original parent using CodonCode Aligner software (CodonCode Corporation, USA).
Clones were verified by sequencing.
All target clones were verified by sequencing.
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