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The infectivity of the evolved clones was increased to 1.7×104-fold as compared to that of fd-RP, which corresponded to an increase in fitness of 9.7.
The mean number of detected species was statistically higher when the number of sequenced clones was increased (6.0±4.0 species for 24 sequenced clones versus 9.0±3.1 species for 40 sequenced clones) (p = 0.05) (Figure 2).
The prevalence of metastasis of the parent, polyclonal, cell line and of its metastatic clones was increased if the cell inoculum was mixed with Matrigel.
Additionally, the size of labelled bulge clones was increased at 180 days after Tam treatment when compared with the earlier tracing time points (days 3 and 8; Figure 2H).
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This advantage is neither due to a prolonged growth period (PTEN clones are increased in size early after clone induction) nor to a complete insensitivity to starvation.
Already 36 hr after clone induction, the PTEN clones were increased in size, and the overgrowth of PTEN clones and the reduction in wild-type tissue were fully apparent after 60 hr, excluding the developmental delay as a main cause of the overgrowth.
These vulnerable code clones are increasing in conjunction with the growth of OSS, potentially contaminating many systems.
Expression of clone G32C4B was increased by up to six-fold in stage 1B cervical cancer biopsies in comparison to the corresponding adjacent normal tissues.
Interestingly, within 14 days of commencement of anti-CD4 treatment, both macroscopically visible metastases in lung/liver were seen, and the frequency of tumor cells cloned from DLN was increased ~30-fold relative to mice receiving control Ig.
In each clone, E-cadherin expression was increased compared to control cells stably transfected with a scrambled sequence (Figure 6B).
We isolated several clones in which DUSP16 expression was increased relative to parental DG75 cells.
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