The majority of analyzable clones were in-frame rearrangements (27 of 30 ATLD clones and 69 of 72 NBS clones).
The morphology of these clones is in sharp contrast to wild-type GFP-expressing clones (Fig. 1b).
The unique T cell clones in the primary lesions are shown in red, the unique T cell clones in the brain metastasis are shown in blue, and the overlapping clones are in purple.
In the T-cell repertoire of a healthy person, approximately 99.6% of the CDR3 clones were in low abundance, with frequency <10 -3 <10 -3
Homozygous targeted hESC clones are shown in blue, heterozygous targeted clones are in red, and untargeted clones are in black.
Twelve clones were in frame with no stop codons.
Most of the questionable clones were in a few large contigs (Table 3).
The genotypic identities (L1; L2) of the regenerated clones are in accordance with their respective phenotypic characteristics.
This clone was in the vector pBluescript II SK+.
For the Nterm 6His tagging, amplification was with TnIFN52 and TnIFN33; cloning was in pIVEX2.4bNde.
