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A library of several thousand clones was generated, with an average insert size of 1.5 kb.
Additional sequence for these clones was generated.
The CCL1 library composed of 19,200 clones was generated with MboI partial digestions.
A library of individual S. marcescens Db10 mini-Tn5Sm mutant clones was generated using standard methods.
After filtering, a subset of 4603 clones was generated for analysis.
Three-dimensional model of clones was generated using the Mimics software from Materialize, Leuven, Belgium.
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Later, deletion clones were generated using sub-cloning strategies involving PCR and restriction digestions (Fig. 1C).
Following manufacturer's instructions, clones were generated by a TOPO® TA Cloning kit (Invitrogen, Carlsbad, CA).
Deletion and point substitution mutant clones were generated using a QuikChange Site-Directed Mutagenesis Kit (Agilent, Clara, CA).
A total of 239 unique clones were generated from 1447 recombinants, and 214 unique sequences were obtained.
McBride et al. (2008) founded populations of viruses from the clones with high, respectively low robustness (how these clones were generated will be described in a later section).
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