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The growth of the clones was assayed by platting 50,000 cells per well in 12-well plates in triplicates and counted at 1 6 days.
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Antibiotic resistant clones were assayed for Tet-repressor expression by transfecting individual clones with TNF-TetO2-EGFP and monitoring EGFP fluorescence by flow cytometry.
Clones were assayed in the absence and presence of efavirenz and nevirapine (obtained from the NIH AIDS Research and Reference Reagent Program) to monitor RT activity and NNRTI susceptibility.
EGY48[p8op-lacZ] was then co-transformed with the different fusion plasmids used in this study and clones were assayed for growth and blue colour onto GR and GRLX media, according to the manufacturer's instructions.
Surviving single clones were assayed for inducible protein expression of TRAIL.
The clones were assayed at identical protein concentrations and binding detected using anti-FLAG and a tyramine-DNP amplification system.
This aspect of the paper could be vastly improved if hundreds of clones were assayed, a statistical treatment was applied, and a map was provided.
Far too few clones were assayed, and the data provided is just text, without tables, a proper statistical treatment, or a map (text and a figure are provided).
CEL7A variants containing from 1 to 19 mutations were expressed in A. niger for screening, and approximately 100,000 clones were assayed for improved stability.
Ninety-four clonesones were assayed against L1210 subcloned cells, DTIC-treated and untreated, and against different murine tumours (syngeneic or allogenic).
All five scFv clones were assayed as both monovalent scFv and bivalent scFv-AP at equimolar concentrations (1 × 10-7 M) on HER2 coated wells.
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