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(Mr. Lucas's "Clones" was also shot digitally and received this week's most up-to-the-moment digital projection at the Palais, perhaps the best place in the world to see a movie).
The function of the ASGPR of these clones was also confirmed by ASOR binding and internalization assay.
The gain of invasiveness of MDA-B02-ATX clones was also observed when compared to parental cells and MDA-B02-NPP1 clones, placed either in absence or presence of doxycycline.
Chromosomal location of clones was also determined from Flybase.
The final single scaffold of the six BAC clones was also assembled with the SeqMan.
The number of clones was also variable among samples and animals.
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These clones are also added to the ARB pool.
In addition, type-4 clones are also detected by the tool CloneManager.
These common clones were also less infected than expected by chance, given the infection frequencies in the lake.
These positive clones were also verified by the amplification of btd specific DNA amplicon using colony PCR assay (Additional file 1: Figure S1C).
The PCR product was digested with BamHI and HindIII, then ligated into the pET-30a vector and 30 different positive clones were also sequenced.
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