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Next, we generated per-deficient mutant clones to test whether the defect associated with PER loss was cell autonomous.
We also included both parental clones to test or support any genetic model that might arise from the obtained data.
We then used these clones to test for possible effects of the introduced synonymous mutations on both early (infectivity) and late (production) stages of the HIV-1 replication cycle, in human embryonic kidney 293T cells (HEK293T).
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To examine whether the cells within the SKP spheres could undergo self-renewal by serial cloning in vitro, eight HGPS-SKP spheres were cloned to test their capacity for self-renewal.
Secondary screening to confirm hits (defined as those variants producing greater luminescence compared to that of the parental clone) and to test combination sequences was completed using a similar protocol but in manual fashion and in triplicate.
For wsp, PCR products were first sequenced directly and, if direct sequencing failed three times (or repeatedly generated sequences with multiple peaks), the PCR product was cloned and 6 10 different clones were sequenced to test for the presence of multiple Wolbachia strains.
As we know nothing about potential previous clones our ability to test the so-called "Frozen-Niche-Variation model" [ 73, 74] is rather limited in this case.
Murine and human EWS/FLI-1-transformed clones were used to test the effects of the KRAB/FLI-1 hybrid protein on the transformed phenotype.
These colonies were propagated as clones in order to test their expression of the monoclonal antibody ( 791T /36 -defined antigen and their resistance to methotrexate (MTX) by comparison with parental cells.
These downstream analyses, in combination with M-CGH analysis will reduce the number of genes required to be cloned in order to test the immune response of their encoded proteins.
The PCR products of six individuals, two belonging to the BAR_MON population and four from the SIC_MAZ population, were cloned and sequenced to test the conflicting haplotypes obtained with PHASE.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com