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The mating system was investigated using manual pollination tests on six clones, open-pollinated seeds were collected from four other clones to study gene flow and paternity analysis was done on progenies using microsatellite genetic markers.
Whilst this is useful and indeed can indicate potential clones to study in more detail (c.f. [ 27]), to more accurately define what is happening at the molecular level, actual gene identifications are required.
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In the present study, fliC gene was cloned to study its biological implications.
Like others [ 14- 16] who found marine sediments were efficient inocula in seaweed AD we adopted, instead of gene cloning, to study complex microbiota in inocula from diverse locations in order to select efficient seaweed hydrolysers for AD, and found that a mixture of all the inocula (including North Ronaldsay sheep rumen and human sewage AD slurry microbiota) was most productive.
This substantially limits the power to trace the frequencies of single clones and to study clonal structure in general.
Other groups using the T-cell cloning assay to study BD-exposed workers in the Czech Republic also failed to find an increase in HPRT MF, even though the level of BD exposure was similar to that used in the studies in the Texas plant (Albertini et al. 2001; Tates et al. 1996).
We generated cell lines from the homozygous WASP-knockout clones in order to study the role of WASP in MK physiology.
In this work, we analyzed carnivore microsatellite clones from GenBank to study their association with interspersed repeats and elucidate the role of the latter in microsatellite genesis and distribution.
The second "licence to clone", this time to study motor neurone disease, was granted a few weeks ago to a consortium including Ian Wilmut, the cloner of Dolly, and researchers at the Institute of Psychiatry in London.
Next, the cell electrophoretic mobility (EPM) of each clone was measured to study the charge-related properties using microcapillary electrophoresis chips according to Omasu's methods [ 37].
These collections could provide the information necessary to reconstruct their own history - the sequence of changes introduced into the lineage during the development of the clone - and to study affinity maturation in detail.
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