Sentence examples for clones to define from inspiring English sources

The authors use this system in combination with MARCM clones to define the temporal periods and spatial properties of R-cell filopodial dynamics during target acquisition and stabilization in the optic lobes.

These six CFSs have been previously mapped by fluorescence in situ hybridization (FISH) with BAC and PAC clones to define the regions of highest breakage, designated as CFS sequences and the flanking NCFS sequences (12– 12).

In the case of TMRA, we also fully sequenced the cDNA clone to define its 3'-end and establish the presence of a polyadenylation tag (polyA).

Transcript screening investigations have traditionally been led by sequence analysis of cDNA clone collections to define the identity of hybridisation probes included on microarrays for expression profiling [ 1].

As both DMA1 and DMA2 satellite types are found at Aye-Aye centromeres, we further characterized all respective monomers (DMA1, 59 monomers; DMA2, 34 monomers) in the CENP-A ChIP-cloned library to define intra- and intersatellite sequence relationships.

Using the cultivable clones we sought to define the taxonomic status of the Burkholderia SAR-1 metagenome.

Consensus sequences derived from 5' and 3' reads of the same cDNA clone were linked to define 14,471 transcripts.

To help epidemiologic investigation and to define clones, i.e., groups of genetically similar isolates descending from a common ancestor, a variety of typing methods have been used, including pulsed-field gel electrophoresis (5, 6 ), single nucleotide polymorphism typing (7 ), and multiple housekeeping and virulence gene sequencing (8, 9 ).

Provided that the same value can be used to define clones within species of Mannheimia, it indicated that all flocks investigated have been exposed to several introductions of Mannheimia spp. over time or that several clonal lineages have evolved within each flock.

This technique has been previously established to define single clones while avoiding the overlapping of two separate clones (Levison and Goldman, 1993, 1997).

These results reinforce the importance of characterizing GAS using PFGE and the SmaI/Cfr9I endonucleases to define GAS clones.