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The major limitations of this system are the efficiency of isolating hybridoma clones, the process of stabilizing these clones and optimization of hybridoma cell secretion, especially for large-scale production.
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Despite all the excitement and apprehension that surrounds cloning, the process remains remarkably inefficient: Just 1% to 5% of cloned mammals survive.
Cloning, the process of generating a genetically identical copy of a cell or an organism.
This sequence is used to query a BES database to find a minimally overlapping clone; the process is then repeated iteratively (Venter et al. 1996).
If you're lucky, you'll have created a "bad" clone in the process of making several clones.
Dolly the sheep was cloned using the process of somatic cell nuclear transfer (SCNT).
To correct the errors induced by the polymerase and evidenced in the cloning process, both the PCR product and its corresponding cloned sequence were assembled.
The first difficulty, which is intrinsic to the cloning process whatever the trait, is the lack of precision in positioning QTLs in mapping populations or, to a much lesser extent, in association panels.
During the cloning process, the genome sequence of G. raimondii (D5) was released [ 47], which confirmed the identity of omega-3 genes we had identified in this organism.
Rosen's essay on the potential consequences of human cloning speculates that the clones themselves might oppose the process.
The essence of the cloning process is that the nucleus of an adult cell is reprogrammed to its embryonic state by the cytoplasm, or inner fluids, of an egg cell.
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