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In some of the stably transfected ERα-expressing MDA-MB-468 clones, the expression of the slug gene was augmented in the absence of E2.
At this developmental time, in most of the induced dTIEGS14 clones the expression of Dpp/BMP2 target genes was nearly unaffected (not shown).
To assess apoptotic cascades in the PNT1a and PC-3 stable clones, the expression of procaspase-9, cleaved caspase-3 and cleaved PARP were measured by Western blot.
Clones that expressed the reporter shortly after integration were excluded from the analysis, because in these clones the expression could have resulted from rearrangement of the construct during integration or from integration of the construct in tandem arrays.
Upon nocodazole (NDZ -mediated tetraploidizatioNDZ -mediatedary Figure 1) and selectetraploidization and p53−/− tetraploid cloneSupplementarysion oFigured-Centrin remained constant, thereby allowing for the detection of supernumerary centrosomes, which are known to arise in p53−/− tetraploid cells.
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The sequences of the entry clones and the expression clone were confirmed by sequencing.
AS cloned the expression constructs.
EW and KT cloned the expression constructs.
Several G418-selected clones showed the expression of the Flag-tagged eIF3f with different efficiencies.
Clones for the expression of CFP alone or YFP alone were also published previously [20].
This was cloned into the expression plasmid pET 26b) and expressed in BL21 DE3) Escherichia coli (E. coli).
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