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Among the SNP-positive clones, the average numbers of SNPs was 2.1 to 3.1, with no significance differences among the cell lines.
The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb.
Panel b: CHEF gel electrophoresis and Not I digestion of random selected FAC clones, the average insert size was estimated at ~110 kb.
Based on the analyses of a subset of clones, the average insert size was estimated at 90 kb, ranging from 50 to 130 kb.
Among the 59 sequenced full-length clones, the average lengths of the 5'- and 3'-untranslated regions (UTRs) were 127 bp and 203 bp, respectively, and the longest 5'-and 3' -UTRs were 486 bp and 480 bp, respectively.
Seki et al. (in RIKEN Plant Science Center) determined full-length sequences of 21 005 RIKEN Arabidopsis Full-Length (RAFL) cDNA clones the average size of RAFL cDNA inserts was estimated to be 1445 bp (Seki et al., unpublished results).
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After removing all the non-ES cells from each clone, the average number of cells per P-clone is 151,217 cells, ranging from 58,770 to 196,069 cells.
For each F2 clone, the average trait values were used in the linkage mapping.
In the HooCam09 clone, the average pairwise nucleotide identity among these pairs was 99.8%.
Because the average insert size was 155 kb and the average valid bands were 80.2 per clone, the average size per band was estimated to be 1,933 bp.
For each BAC clone, the average ratios that deviated significantly from zero were considered to be abnormal (± log2 0.25), and almost all spots were within the area between the cutoff lines (± log2 0.25) for male/female DNA samples.
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