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Although the available EST libraries for L. bicolor contain approximately 20K clones, the amount of sequence data from the single transcriptomic data set generates 30-times more sequence data than the combined EST reads.
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(The hybridization signal for the polylinker fragment was subsequently used by DArTsoft to determine for each clone the amount of DNA spotted on the array; see next paragraph).
The polylinker fragment was used as a reference to determine, for each clone, the amount of DNA spotted on the array [ 23].
For each variant and WT GCKR clone, the amount of Myc-DDK-tagged protein was measured and normalized to alpha-tubulin via densitometry.
In contrast to the Lec15.2-LG clones, all the B421-LG clones stably overexpressing Large maintained production of abundant functional glycans after 4 months culture (the same period for Lec15.2 clones), although the amount of functional glycans were about 50% of that in Pro5-LG cells (Fig. 2).
As we had anticipated, there is a lower percentage of mutant clones as the amount of the first flanking primer increases, presumably because residual first flanking primer is competing with the megaprimer in the second PCR, thereby amplifying the wild-type sequence.
Not that there's anything objectionable about her personality or the music, but the 21-year-old north Londoner has inspired so many dreary clones that simply the amount of copper fringes on display nowadays is enough to give her an air of tired omnipresence.
The term "insertion frequency" is defined here as the number of LTR sequences identified in a PAC or BAC clone divided by the amount of DNA in the clone.
Pure subtractive methodologies are of limited use due to the need for a large quantity of mRNA to drive hybridization to completion as well as the difficulty in cloning the tiny amount of cDNA remaining after hybridization.
Cloning also reduces the amount of time needed for breeding.
Sensitivity analysis suggested that fruit set and resulting measures of productivity such as fruit mass and viable seeds per fruit were sensitive to parameterization of blueberry genotype or clone size and the amount of blueberry plant cover in a field.
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