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Together, these data indicate that RNAi clones that increase lifespan and induce nhr‐57 expression levels do not necessarily act through HIF‐1.
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RNAi clones that increased intestinal gcs-1p::gfp expression in prdx-2 mutant.
Some authors 70– 72 suggest that the prompt switch to another non-cross resistant agent may reduce the risk of development resistant clones that increases over time.
In the case of serotype M1 GAS, an apparent episode of generalized transduction contributed to the evolution of a new, unusually virulent clone that increased dramatically in frequency since the mid 1980s [56].
To verify whether the fluorescence intensity of bacterial PHB stained by LipidGreen1 agreed with the intracellular PHB contents, we selected mutant clones that exhibited increased or decreased fluorescence intensities compared to wild type phaC and analyzed the PHB contents with GC.
However, we were unable to isolate clones that expressed increased levels of NAT1 protein, possibly due to the instability of the mutant protein which is rapidly poly-ubiquitinated [5].
The resulting clones that exhibited increased Stat1 expression were three- to five-fold resistant to cisplatin and AMD473 as compared to the parental cells.
Using feeding RNAi clones from a library of C. elegans coding sequences (Fraser et al. 2000), we found a set of clones that significantly increased the frequency of unpaired GFP signals in the pachytene region of the gonad.
The results of [H]-thymidine incorporation of wild-type and MXI1-transfected U87 clones confirmed that increased Mxi1 expression causes a decreased proliferation rate of glioma cells at different time points.
However, unlike the genotypes studied here, Weih and Nord [16,34] demonstrated an inverse relationship between leaf area and root biomass in six willow clones, suggesting that increased shoot productivity with increasing leaf area occurred at a cost of reduced below ground biomass allocation.
The clones that showed an increase in xylanase activity of at least 10% in the presence of xylose were submitted to nucleotide sequencing (see Table 1; Fig. 1).
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