Sentence examples for clones that failed from inspiring English sources

This success rate dropped to 80% when second isolates of the failed clones were sequenced, raising questions about the likelihood of success of sequencing additional isolates for clones that failed after two attempts.

Only clones that failed the CDS region evaluation underwent BLAST search against all available GenBank records, and were re-evaluated using matching BLAST hits in pairwise alignments, allowing us to rescue ∼5% of the clones.

Interestingly, we found that all of the clones that gave bone formation expressed high levels of BMP-6 mRNA, whereas all of the clones that failed to generate bone were low producers of BMP-6.

Paired reads were assembled using phrap and all clones that failed to assemble, formed contigs less than 1250 bp in length or contained less than 80% Q20 bases (or 70% for nasal, saliva and dust samples) were removed from further processing.

Clones that failed to meet these criteria were discarded from further analysis.

Even those clones that failed to maintain the U5-IR stem were able to serve as templates in this assay.

The phenotypes of complete loss of human RECQ1 are likely to be more severe than those observed, particularly in the case of shRNA selection, since that protocol presumably selects for clones that fail to completely silence RECQ1.

The morphological effects we observed were not artifacts related to EGFP expression as the 2 11 clone that failed to express EGFP presented the same variations of morphological parameters upon stress (data not shown).

A CBS clone that fails to upregulate CaSR in response to Ca2+ also fails to demonstrate growth reduction or induction of differentiation under the same conditions.

Array production – All A. burtoni cDNA clones (including 1185 that failed the gel analysis above) and 120 control clones were spotted in duplicate arrays onto NaOH cleaned, poly-lysine (Sigma) coated slides using the 16-pin format on an OmniGrid-100 arrayer (GeneMachines).

The leptin gene was isolated by Friedman's group, through positional cloning from ob/ob mice that failed to produce leptin and displayed extreme obesity and hyperphagia.