Sentence examples for clones that fail from inspiring English sources

The phenotypes of complete loss of human RECQ1 are likely to be more severe than those observed, particularly in the case of shRNA selection, since that protocol presumably selects for clones that fail to completely silence RECQ1.

Enlarged and abnormally developed kidneys were also evident in some of the six live-born Finn-Dorset clones that failed to survive to 3 months of age in the current study (Supplementary Table 6).

This success rate dropped to 80% when second isolates of the failed clones were sequenced, raising questions about the likelihood of success of sequencing additional isolates for clones that failed after two attempts.

Interestingly, we found that all of the clones that gave bone formation expressed high levels of BMP-6 mRNA, whereas all of the clones that failed to generate bone were low producers of BMP-6.

Only clones that failed the CDS region evaluation underwent BLAST search against all available GenBank records, and were re-evaluated using matching BLAST hits in pairwise alignments, allowing us to rescue ∼5% of the clones.

Paired reads were assembled using phrap and all clones that failed to assemble, formed contigs less than 1250 bp in length or contained less than 80% Q20 bases (or 70% for nasal, saliva and dust samples) were removed from further processing.

Clones that failed to meet these criteria were discarded from further analysis.

The second class of cDNAs corresponds to 1221 MGC clones that failed to clone during the first human ORFeome project.

Even those clones that failed to maintain the U5-IR stem were able to serve as templates in this assay.

After culture in N2B27 medium for a further 7 10 days, ESC clones that failed to differentiate were isolated and subject to further analysis.

BLAST results showed that except for 4 clones that failed to sequence, probably due to sample cross over contamination, all fosmid end sequences hit the assembled genome at 34 to 45 KB apart in the right orientation giving an overall high efficiency of library construction and quality.