Sentence examples for clones that contained from inspiring English sources

Following G418 selection, clones that contained the knock-in were identified by long-range genomic PCR as previously described [3].

After substracting the amount of clones that contained dimers, the overall number of correct clones increased from 87.5% to 91.5%.

Correctly targeted ES cell clones that contained "tri-loxed" Ate1 allele (Figs. 2C, D) were identified using Southern hybridization and PCR.

Two plasmid clones, that contained 14 informative sites disbursed over the amplicon, were mixed in equal proportions and diluted to 10,000 templates and 100,000 templates before PCR amplification and UDPS.

All clones that contained a PEG10 specific insert started with an expected polymeric run of Gs (complementary sequence of the adapter) and continued with ACACGCGCTTCAACT..On the genomic level, 24 30 nucleotides upstream of this sequence, there is a typical TATA-box sequence (Figure 1A).

Affinity matured clones that contained a TAG amber stop codon in the mutated CDRs (coding for glutamine in suppressor strains and stop in non-suppressor strains), were backmutated to CAG by using PCR backmutation primers as specified in Table 5 and essentially the same assembly procedure as described above.

Second, cosmid clones that contain wild-type DNA inserts spanning the deletion in ga1-3 complementhe the dwarf phenotype when integrated into the ga1-3 genome by Agrobacterium tumefaciens-mediated transformation.

The genetic control of translocation formation and the chromosome complements of the clones that contain translocations suggest that translocation formation occurs by single-strand annealing (SSA).

The study of protein function often demands high quality plasmid clones that contain the relevant open reading frames (ORFs) in a format compatible with protein expression.

In this system entry clones that contain a fragment of DNA of interest flanked by specific attL feet are first generated.

The lengths of our two longest clones that contain the entire Dlk1 ORF are consistent with the size of the 4.5 kb form of alternatively polyadenylated Dlk1.