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We investigated the requirement for Esg in these stem cell tumors by creating esg G66B null mutant MARCM clones that also expressed Notch RNAi.
In contrast, Nanog-GFP fluorescence was not visible in iPS-like primary clones that also formed in lamin A overexpression MEFs.
Third, substantial differences in genome size were always observed among clones that also differed in both their ITS1 and COI sequences (e.g. the two MNCHU clones), suggesting that they belong to reproductively isolated populations, rather than the same population.
Overall, our data clearly indicate that the antibiotic susceptibility of contemporary Ng isolates is dramatically decreasing, mainly due to the spread of international MDR clones that also show reduced susceptibility to the standard therapeutic options CRO and AZT [ 37].
The nuclei in the clone lacked Sal staining (similar to DB cells), and in contrast to clones that ectopically over-express kni in an otherwise normal genetic background, the clones that also down-regulate Notch do not sort out, but appear to integrate with their neighbors without forming a bulge.
Furthermore, the second most common hit in our pool analysis, a transcription factor MEIS1, was only selected in IMR32 cells, and in clonal analysis was only seen in clones that also had insertions in ABCB1, implicating a possible role for MEIS1 in modifying ABCB1-mediated resistance, rather than inducing resistance alone.
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Poke, a Snapchat clone that also lets people send disappearing messages, failed to gain traction when released at the end of 2012.
With regard to JNK and p38, CP-55,940 induced a small (150 170% of basal) but significant increase in the phosphorylation state of these kinases only in the CB2-high clone that also lasted throughout the 30 min incubation period (Figure S2a,b).
The meiosis observations on P. Lilin are consistent with previous work [ 10, 11] on this same clone that also drew the conclusion of an inversion.
This is part of a collection of tools, called Clone Mapper, that also allow clone verification and sequence retrieval.
We isolated a single partial ORF clone (XL1) that also lacked the 3' end.
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