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For two clones showing a low-level of resistance for Danusertib (IC50: 2.1 and 2.8 µM, respectively) a reduced inhibition of P-CrkL was found when compared to Danusertib-sensitive cells.
We defined "unique paired-end clones" as paired-end clones showing a single match at each BES.
We first compared the number of events, which was defined as percentage of clones showing a gain or loss.
Consequently, we re-ran the analysis, taking into account only the five clones showing a unimodal distribution.
ANOVA was performed on normalized data enabling us to keep 818 clones showing a significant relative expression level change (ratio of 1.2) between the two developmental stages.
The two well-established transformed root cultures (HR E1.5 and HR E1.16) were clones showing a medium growth rate and high stability with regards to their FAs production.
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Two independent knockdown clones showing an approximately 70% decrease in β-catenin levels and a control LacZ shRNA were analyzed.
Clones showing an induction of luciferase activity in the absence of Tet were transfected with the WTPTEN cDNA construct cloned into the pTRE2Hyg vector (TetWTPTEN).
Positive clones showed a band at 1.7 kb (Additional file 2: Figure S2).
Some clones exhibited a uniform production across replicates, implying low susceptibility to soil heterogeneity; other clones showed a high inter-replicate variation.
In the GAC packed MFC, clones showed a great similarity to Comamonas, and the amount of Comamonas accounted for 41% of the population.
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