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Although most clones show normal aPKC localisation, a significant proportion has reduced levels of apical aPKC and a few lack apical aPKC entirely, and the original conclusions of this paper are therefore unchanged.
We previously reported that clones of Psc-Su z 2 Psc-Su z 2nt cells in imaginal wing discs shor a tumor phenotype caused by hyperproliferation of the mutant cells, but that Sce or Pc mutant clones show normal cell prolineratimaginalchle et al., 2001; Oktaba et al., 2008).
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The vast majority of baz EH747 and baz XR11 FE clones showed normal polarization of the FE and maintained aPKC, Par6, Crb and Sdt localization.
However, all of these clones showed normal induction of the wild-type GPXH gene, indicating that under these screening conditions no gid mutants can be isolated.
We selected 16 clones and clone 6 showed normal embryoid body formation and cardiomyocyte differentiation.
Dg and ampk mutant clones still showed normal polarity after treatment with these drugs, though many egg chambers degenerated at stage 7/8 because drug-induced starvation activated a nutritional checkpoint that blocks vitellogenesis (Drummond-Barbosa and Spradling, 2001) (supplementary material Table S1).
Both proteins show normal localisations in small clones with defective epithelial organisation, whereas aPKC and Par-6 levels are already elevated in small clones that still retain a normal shape (Fig. 4H-K).
Gp150 mutants show normal host colonization [27].
Selection with doxorubicin frequently resulted in reduced topo II alpha mRNA and protein levels, whereas clones selected with vincristine showed normal levels of topo II alpha.
In some of the cell clones from patient S2, the degree of hypomethylation at the CpG sites M2 to M4 was almost 0%, while other clones from the same patient showed normal methylation values at these sites (Table 3).
Positive clones (showing signals in normal cell DNA and missing in metastatic cell DNA) were sequenced.
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CEO of Professional Science Editing for Scientists @ prosciediting.com