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The emergence of multidrug-resistant CA-MRSA clones and the detection of PVL toxin genes in clones previously reported as PVL negative is a major public health concern.
In this study, the average UDPS reads exceeded 2,000 for core and E1 and E2 proteins, which is much larger than those clones previously reported in other studies.
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35 isolates were considered to belong to the same epidemiological prevalent clone previously reported [4], [5], [9], [28] and 11 strains were consistent to be of different origin.
In addition, five resistant strains of the Sweden15A-25 clone, another multiresistant clone previously reported as susceptible (28 ), were found (Table 3).
A survey and analysis is made of all available ω-gliadin DNA sequences including ω-gliadin genes within a large genomic clone, previously reported gene sequences, and ESTs identified from the large wheat EST collection.
A major problem with our study is the collection of strains used, since they were isolated only in one center and only 01 strain was resistant to polymyxin B. Nevertheless, the evaluation of clonality showed that only 29% of isolates belonged to the endemic clone (clone A) previously reported in our hospital and these strains were excluded of the comparison of analysis of susceptible methods.
The observed variability was likely due to the variation in gp120 sequence across the panel of HIV-1 clones as previously reported by Li et al. using both monoclonal antibody and soluble CD4 inhibitors [16].
This is beneficial because it prevents developing subtractive libraries with larger capacities of clones as previously reported [ 4, 5, 8, 11- 13].
In contrast, seven of the nine lines tested that express cas9 under nanos (nos ) regulatory elements did not have detectable somatic activity when crossed to U6 3-gRNA-e [ FigU6 3-gRNA-e other two lines (CFD3_nos and CFD8) resU6 3-gRNA-eall somatic mutant clones as previously reported (Port et al. 2014)].
The genes encoding ProcA1.1G 1E and ProcA4.3 were cloned from previously reported vectors and inserted into multiple cloning site 1 of the pRSFDuet-1/procM-2 vector using the EcoRI and NotI restriction sites.
The details of this cloning were previously reported by Chakraborti et al. [ 30].
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