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Figure 3C shows clones of various sizes in terms of cell number.
We prepared deletion clones of various lengths by treating HooCam09, HylCam15, and NomCam1B3 with exonuclease III and then with Mung bean nuclease.
We have generated GSC clones of various genotypes examined in this study to show that defective COC/spindle orientation results in a higher frequency of symmetric stem cell division (added as Figure 6).
Seven CHO-IGF-E5 clones of various production levels (as per initial spot blot screening) were seeded at 1.0 × 104 cells/ml in semi-solid medium containing anti His Tag-FITC antibody in duplicate wells of a PetriWell24 plate (Genetix) and, in parallel batches at 1.0 × 106 cells/ml in liquid growth medium.
It is not possible to utilize undifferentiated TGFP EpiSCs as a negative control for FACS as they also express significant levels of both T BRA) protein and the TGFP reporter under self-renewing conditions (i.e. in FGF2 and Activin A) in line with previous reports (Tsakiridis et al., 2014 ; Kurek et al., 2015 ). Figure 3C shows clones of various sizes in terms of cell number.
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These results suggested that the vector system is useful for cloning of various foreign HIV-1 env genes and the recombinant envelope glycoproteins effectively incorporate into MuLV particles.
In addition we provide a restriction map of the murine wt1 locus which should prove useful for the cloning of various constructs designed for the generation of mouse models.
The cloning of various deletion constructs, expression, and purification has been described previously [28], [56], [65], [66].
The pVITRO2-blasti-mcs plasmid containing the gene encoding Tat, pVITRO2-blasti-mcs-tat, was considered as an acceptor vector for the cloning of various genes encoding HA proteins of interest into the second MCS by using the In-Fusion 2.0 PCR cloning kit (Clontech Laboratories , Inc.
This influence of annealing temperature of the low stringency cycle was confirmed by repeated experiments for the cloning of various genes and T-DNA insertion loci.
Therefore, we are currently cloning receptors of various steroid hormones, and steroid and xenobiotic receptors (SXR), from various animal species, including alligator, quail, and various fish species, in order to find species that are sensitive to EDCs.
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