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Sequencing of multiple clones of three separate DNA fragments spanning 40% of the genome also indicated that DEN-4 propagated in Vero cells contained a higher number of mutations than the virus growing in MRC-5 cells.
To determine whether ft is also required for follicle-cell differentiation and oocyte polarity, we generated follicle-cell clones of three separate alleles of ft: ft422 [58], ftG-rv [59], and ft8 [60], but no oocyte polarity or follicle-cell fate defects were detected (data not shown).
Ten BAC clones of three Oryza species were sequenced with an ABI 3730 automated sequencer (Table 1).
We used single representative clones of three species of Salix: S. viminalis, S. dasyclados and S. cinerea.
Clones of three independent mutants were enriched by seven rounds of selection and the mutant which was most frequently obtained (FA11) was further investigated.
Here, we examine how systemically produced plant volatiles, in response to local herbivore damage, differentially attract a key omnivorous predator, Anthocoris nemorum (Heteroptera: Anthocoridae), to single clones of three species of Salix: S. viminalis, S. dasyclados and S. cinerea.
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Unlike Dolly, however, such clones are derived from a single zygote, or fertilized egg, and thus they are clones of one another, rather than clones of another individual.
The plantations contain hybrid clones of two Eucalyptus species, Eucalyptus grandis W. Hill ex Maid and Eucalyptus urophylla S.T. Blake.
To investigate viral factors, other than dominant mutations that could be involved in fitness increase, we quantified the heterogeneity of mutant spectra, by sequencing molecular clones of four genomic regions of the initial clones and the corresponding passaged populations.
The SA and SB pools contain clones of six and eight 384-well plates, respectively.
In the present study, the full-length cDNA clones of seven P450s and seven UGTs were obtained.
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