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The naive population typically contains many clones of small number.
Thus, the presence of clones of small cells within the plane of the normal wing blade may be a primary defect that contributes to the observed phenotypes.
awd mutant clones exhibiting normal epithelial integrity are most often observed in clones of small size (<10 cells; unpublished observation).
Single-cell cloning by limiting dilution of the heterogenous LM38-LP cell line resulted in the initial isolation of several clones of the large spindle cells with large nuclei, as well as some clones of small spindle cells and a few clones forming epithelium-like islets.
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EBV miRNAs were first identified by Pfeffer et al. via cloning of small RNAs from a Burkitt's lymphoma cell line latently infected with EBV [6].
However, limited molecular cloning of small RNAs derived from several positive-strand plant RNA viruses has revealed a strong strand bias towards the genomic sense strand, which could argue against the idea of genome-length viral dsRNA as being the sole precursor [15].
The expression was indicated by direct cloning of small RNAs from 17-day old female An.
This could be attributed to the biased cloning, although what causes the biased cloning of small RNAs is unknown.
Finally, cloning of small amounts of sample DNA is often inefficient and studying single cells is therefore not possible at the moment.
Identification of miRNAs in different plant species has been based on the cloning of small RNA fractions and/or the prediction of miRNA genes in genomic sequences based on sequence similarity [ 1].
Construction of future libraries for use in yeast will have to combine the cloning of small fragments corresponding to single gene size with an efficient transformation protocol to accommodate the larger colony numbers required to represent the same soil population.
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