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Furthermore, since genome editing was confirmed for more than 50% of clones obtained from transient selection with G418, our system for generating gene knock-out could be applied to some of the other Dictyostelia species.
Sequence analysis of the clones obtained from 16S rDNA clonal library showed affiliations with the genus Moorella sp. CF4 ranging from 94% to 97% identity.
Here, we report two novel members of the temporin family, named temporin-1Ee (FLPVIAGVLSKLFamide) and temporin-1Re (FLPGLLAGLLamide), whose biosynthetic precursor structures were deduced from clones obtained from skin secretion-derived cDNA libraries of the European edible frog, Pelophylax kl.
The 13,200 clones obtained from both libraries were then submitted to fosmid DNA extraction in pools and subsequent pyrosequencing.
Phylogenetic affiliations and number of bacterial clones obtained from retained and suspended fractions in MFC operated for 30 days at pH 4.0.
Clones obtained from the same sample proliferated at similar rates.
Clones obtained from this transfection were named BF-LF17.x or BF-LF18.x.
Molecular env clones obtained from the monocytes of six patients were compared to the env clones obtained from CD4+ T-cells purified by negative magnetic selection from the same patients.
The unc-10 and rab-3 fusion constructs were made from C. elegans cDNA clones obtained from Yuji Kohara.
The R46X mutation was observed among the cDNA clones obtained from total and CD4+ PBMCs and the Jurkat cell line.
The number of clones obtained from a single fiber was 2.4±0.7, confirming the absence of contaminant cells (Fig. S2).
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