Sentence examples for clones lacking the from inspiring English sources

Some selected clones lacking the insert can often be found from impurities produced by restriction endonucleases or unspecific amplification products further ligated into the vector.

Likewise, recovering clones without the insert's sequence coincides with numerous cases where plasmid vectors, having positive selection systems, have presented several recombinant clones lacking the insert (Ma et al. 2014; Pierce et al. 1992).

Clones lacking the major promoter of MALAT1 display stronger effects on expression.

The exact effects of these insertions will be studied experimentally after establishing BAC clones lacking the LTR fragment.

Similar to results in bulk cells, clones lacking the upstream promoter region of MALAT1 do not display a strong reduction in RNA levels (Fig.  6a).

However, basal R-Luc activity in the targeted intermediate was more than 300-fold higher than in the clones lacking the Zeocin selection cassette.

If subsequent plasmid analysis reveals that clones lack the desired mutation, increase the digestion time to three hours.

However, the usefulness of EST clones is limited; because many EST clones lack the complete sequences of mRNAs, they cannot be used to reveal the primary structures of entire genes and encoded proteins [ 10].

Kaiser et al. reported that cells expressing a SARM clone lacking the N-terminal region did not exhibit apoptosis.

The NT (residues 1 604) and IBC (residues 224 604) of rat IP3R1 were amplified by PCR from the full-length receptor clone lacking the SI splice region (GenBank® accession number GQ233032.1) as described previously [ 33].

N-terminal fragments of IP3R1 (IBC, residues 224 604; NT, residues 1 604) were amplified by PCR from the rat IP3R1 clone lacking the S1 splice site and ligated into pTrcHisA vectors for expression of N-terminally tagged His6 fusion proteins as previously described (Rossi et al., 2009).