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The 49 secreted proteins which accumulated early in resistant clones indicate that this compartment is active in HR initiation or other early resistance mechanisms (Table 3).
Taken together, the clonal heterogeneity that is frequently present and the broad sensitivity of BRAF V600E-positive clones indicate that broad acting drugs still should be a backbone of the treatment regime.
The graded nature of malignant properties and the differentiation between local growth and metastatic potential among the daughter clones indicate that malignancy reflects a complex moiety of cell properties.
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Sequence analysis of nine positive clones indicated that indeed DNA polymerase genes had been isolated from the library.
The generation of somatic and germ line mutant clones indicates that lin-52 is required for adult eye development and for early embryogenesis via maternal effects.
Further sequencing and analysis of several randomly chosen clones indicated that all of them are mostly likely involved in the biosynthesis of methyl-branched fatty acids.
Sequences from 14 randomly selected phage clones indicated that the distribution of nucleotides and amino acids paralleled with the expected frequency.
The nucleotide and predicted amino acid sequences from peripheral T cells cDNA clones indicated that the genes from cluster 3 are fully implicated in the beta chain recombination machinery.
The frequency of common predicted loss of function mutations varied significantly amongst the clones indicating that heteroplasmic mtDNA variation could lead to a continuous range of phenotypes and play a role in cell to cell, production run to production run and indeed clone to clone variation in CHO cell metabolism.
Sequence analysis of cDNA clones indicates that the liver and islet glucose transporters have identical sequences and, thus, are the products of the same gene.
Further analysis of recombinant clones indicated that sequence length was similar to that for pGEM-T Easy vector; inserts ranging from 350 to 3000 bp being efficiently sequenced.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com