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Flybow 1.1B allows in vivo-description of clones in three independent hues within the same animal (Shimosako et al., 2014), which offers high efficiency.
For example, we observed a field effect in tumors harboring TP53 mutations and the presence of subclonal PIK3CA mutations or of multiple mutated clones in three tumors, probably resulting from their evolution.
We therefore determined the fitness of the evolved clones in three environments: low (1 mM) sucrose, low monosaccharide (1 mM glucose plus 1 mM fructose, the hydrolysis products of 1 mM sucrose), and high (80 mM) glucose.
The number of copies of the docking site was determined for each GFP-expressing clone by ddPCR, and clones carrying a single docking site per cell (6 40% of GFP-expressing clones in three experiments with Kc167, 20 30% in two experiments with Sg4) were expanded, saved as frozen stocks, and used for further analysis.
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Le Guin often explores alternative sexuality in her works, and has subsequently written many stories that examine the possibilities SF allows for non-traditional homosexuality, such as the bisexual bonding between clones in "Nine Lives".
Starting from the MGC resource, we created protein expression ORF clones in two different formats for over 3400 human genes, HFLEX7000, making them the largest contribution of fully sequence verified ORF clones to the ORFeome Collaboration (www.Orfeomecollaboration.org ).org
We pooled 300 transformant clones in six pools, each containing 50 colonies, isolated plasmid DNA from these pooled samples, and screened the DNA by PCR for suitably located transposon insertions (Figure 3A).
The 6,389 ESTs were sequenced from 5,952 different cDNA clones in six developing xylem cDNA libraries.
All RFLP markers hybridized to clones in two or more contigs.
The differentially expressed clones in two cDNA libraries were selected and sequenced.
Even the majority (239/363) of microsatellite markers could generate amplicons from clones in two or more contigs.
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