Sentence examples for clones in either from inspiring English sources

Transient transfection of minigene constructs in HEK 293 cells was used for genomic and small molecule compound high-throughput screens (HTS) described below, due to superior luciferase signal when compared to stable clones in either HEK 293 or C2C12 cellular backgrounds (data not shown).

However, none of the BESs of clones in either contig25 or contig821 yielded cowpea EZA1 genes when BLASTed to the HarvEST Cowpea database.

Importantly, we did not find differences between the bulk sample and two single-cell clones in either stock apart from a partial loss of heterozygosity (LOH) in chromosome 21 of the bulk WT-IAH, which is complete in the two isolated clones.

They were cloned in either pGEMT, pGEMT-Easy, pBlueScript or pUC II vectors.

PCR fragments were then cloned in either, Grand Island, NY P221 (Invitrogen) or pENTR-D/TOPO (Invitrogen) to generate gateway (Invitrogen) entry clones.

Four different siRNA sequences were cloned in either pSilencer 1.0-U6 or pSUPERretro.puro expression vectors and expressed in GN-11 neurons following transfections and puromycin selection.

The TnIFN orf was cloned in either pIVEX2.3-MCS or pIVEX 2.4bNde (6HIS Cterm and Nterm fusions respectively) and the resulting plasmids were used to produce recombinant TnIFN.

The most efficient method in identifying novel miRNAs seems to be the identification of potential hairpin forming precursors encompassing the mature miRNA sequence of interest (obtained by cloning) in either genomic or EST sequences.

An additional gene (teashirt, tsh) showed signal in M. abdita but not C. albipunctata (not shown), while the other candidates could not be cloned in either species, or did not show any consistent spatial expression patterns.

Clones growing in either uniform or no nutrient treatments showed no strong increase in the distribution of shoot, root, or tuber biomass, or the number of tubers in the section farthest from the maternal section (where plants were sown) (Fig. 1).

Although a 1 3 vector-to-insert molar ratio was tested to improve the recovery of clones having longer inserts (as recommended for optimal blunt fragment ligation (Rapid DNA Ligation Kit, Thermo Scientific)) as well every ligation regardless of TA vector, such strategy did not improve cloning efficiency in either of the two vectors evaluated here.