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The global transcriptional profiles of these early generation clones have previously been established during multiple array experiments and the results have been confirmed using northern blot and RT-PCR [26].
The 4B2A1 and 7A10B2 T cell clones have previously been described [ 43, 62].
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One PduDlx positive BAC clone has previously been completely sequenced [ 29], Genbank CT030672.
This clone has previously been shown to contain 2 mobile genetic elements, Tn 1545 and "mega" (15).
This clone had previously been shown to transform established cell lines, such as NIH-3T3 cells, but to be unable to transform primary rat embryo fibroblasts [ 20- 22].
However, the existence of such an isoform, for which only a cDNA clone has previously been described, remains to be proven experimentally.
This mutant clone has previously been shown to be significantly more radiosensitive than the parent MGH-U1 cell line and together they provide a valuable study model.
Lambda clones containing IGF2 have previously been mapped to tammar wallaby chromosome 2p [ 27].
However, cloned prokaryotic channels have previously been shown to function in both heterologous expression systems e.g. [5], [6], [20], [24] and in artificial lipid bilayers e.g. [19], [25].
Approaches based on real-time PCR to validate ES positive clones have been previously described, using absolute quantification of NEO, or a relative quantification to detect deletion of the target gene [ 9, 10].
We have previously cloned and expressed in the soluble form the fourth shikimate pathway enzyme of the M. tuberculosis, the aroE-encoded shikimate dehydrogenase (mtSD).
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