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Persistent grass pollen-specific T cell clones have also been demonstrated in vivo [ 29].
cDNA clones have also been isolated from wheat [ 4, 17- 19] and barley [ Hordeum vulgare; [ 20]].
In North America and Europe, most research on LA MRSA has reported on CC398 [ 7, 8] although human clones have also been identified on livestock [ 45].
The gRNA genes corresponding to contigs 878 (1 clone) and 957 (16 clones) have also been found to be up-regulated under PS (Additional file 3: Table S8) and were previously found as up-regulated in metacyclic promastigotes [ 25].
However, shared clones have also been found between blood and synovial B cells and between B cells from different joints [ 58, 59], and synovial B cells are probably a mix of in situ generated clones and clones from distal locations that subsequently migrate in response to inflammation.
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Variation in gene expression between clones has also been demonstrated for the stevor and Pfmc-2TM families, which also exhibit variation in switching rates between isogenic clones [29].
The DNA methylation pattern of iPS clones has also been used to predict the somatic cell from which they were derived.
The selective expansion of specific clones has also been inferred from global measurements such as V region usage (Reuther et al., 2013) or spectratyping (a technique sometimes referred to as the immunoscope) (Russi et al., 2013).
Meanwhile, gene-enrichment techniques such as methylation filtration and Cot-based cloning have also been used to compare G. raimondii, G. arboreum, G. hirsutum, and G. barbadense (B. Scheffler, Workshop communication).
Cloning has also become reality.
Cloning has also allowed us to identify cell lines with more desirable properties such as higher basal insulin secretion and/or better glucose responsiveness.
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