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The clones from all the samples were related to Candidatus Scalindua with more than 90%% similarity.
However, one individual contains clones from all three regions, suggesting that this genomic locus harbors a more complex rearrangement.
In total, 29 clones from all four studied species formed this lineage in NJ analysis based on nucleotide sequences of RT domain.
Despite the variable killing of parasitised cells, over 60% of the T-cell clones from all four animals gave significant levels of specific killing of peptide-pulsed targets.
Clones from all libraries with the exception of PstI+ BanII Library C were used to create arrays in order to genotype several hundred sorghum accessions (data not presented).
We did not observe any overlap of CDR3 amino acid sequences or a preferential Vβ or Jβ gene usage among the top 100 expanded clones from all SLE patients.
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Clade I genes were cloned from all of the clupeocephalans examined.
MALDI MS analysis of purified IgG molecules cloned from all patients on study was used to calculate the differences between theoretical and determined MW caused by glycosylation.
Nevertheless, the shared lack of intact motifs necessary for removing intronic sequences, in mstn2 paralogs cloned from all three subfamilies, suggests that the patterns are indeed conserved among all salmonids.
Plasmids cloned from all libraries were sequenced from both ends of the inserts with standard M13 forward and reverse primers using ABI Big Dye Terminator v3, and reactions were analyzed on the ABI Prism® 3700 DNA Analyzer.
A total of 75 MuERV U3 sequences were initially cloned from all three tissues of both burn and no burn mice, and subsequent multiple alignment analyses identified 31 unique U3 sequences with nine different sizes (346 bp, 384 bp, 392 bp, 405 bp, 406 bp, 433 bp, 556 bp, 600 bp and 601 bp) (see Additional file 1, Figure S1).
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