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The library also contained numerous cDNA clones for both RHL1 and RHL2 that were derived from unspliced precursor mRNAs.
Vajta consults for a company in China called BGI Ark Biotechnology that makes pig clones for both agriculture and as models for human disease.
For experimental validation, a tool has been developed using the proposed LWH approach to detect method/function level clones for both C and Java projects.
In this paper, we have proposed a LWH approach to detect method-level clones for both textual similarity and functional similarity types with the computation of metrics combined with simple textual analysis technique.
In the wing imaginal disc epithelium, homozygous mutant clones for both Psc and the related adjacent Su z 2 gene lead to an expulsion phenotype resembling that of ph mutant clones, while this was not the case for other PcG genes tested, notable Asx, E z), Pc, Pcl, Sce and Scm [22].
We identified cDNA clones for both X. tropicalis and X. laevis ADAM17 from Xenopus EST collections using BLAST searches.
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To our knowledge, these are the first examples of dual promoter vectors that allow for one-step cloning for both bacterial and yeast expression.
Multilocus sequence typing revealed a single major circulating clone for both Hif (sequence type 124; 89/99 isolates, 90%) and Hie (sequence type 18; 21/33, 64%), but no association between type and clinical disease or outcome was found.
Because the analysis uses only BAC clones for which both sets of data are available a much reduced number of BAC clones are included.
PERL scripts were then used to identify BAC clones for which both ends mapped to the same construct, ensuring that the ends were oriented properly to represent the clone (i.e. 1 sense and 1 anti-sense) and that the distance between the mapped ends was appropriate based on the average clone size of the library (TARBAC: avg. length 75 kb, EPIFOS: avg. length 35 kb).
The proportion of fully-sequenced clones was 36.8% for both-ends sequenced clones and 12.9% for 5'-end sequenced clones.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com