Exact(26)
Where False Negative [N] = Actual clones [A] – correctly detected clones[C] which reports the number of clones failed to be detected.
Most of the clones failed to develop, but 22 grew into blastocysts, and 17 of those were inserted into nine mares, including the Haflinger.
Surprisingly, drug resistant clones failed to manifest such drastic changes in PL profiles, demonstrating that the molecular basis of miltefosine resistance lays in distinct biological processes not related to lipid biosynthesis.
Second, another 19.8% of the expression clones failed to produce any observable GFP-tagged protein expression in transgenic animals.
These DR4 and DR5-encoding cDNA clones failed to restore reporter gene activation when FADD was omitted, showing their dependence on this adapter protein for inducing activation of Caspase-8 (Figure 3E, F).
Myogenic clones were distinguished from other clones of the same ectopic stem/progenitor cell population: myogenic clones readily transformed into MHC-positive myotube-like structures, whereas non-myogenic clones failed to express MHC or form myotube-like structures.
Similar(34)
Further, only Importin-α3 is absolutely required for viability: germline clones fail to develop embryos and mutations die at the first- to second-instar transition while importin-α1 and importin-α2 mutants survive through to adulthood [15], [16], [25].
Of note, we have not been able to analyze the phenotypes associated with a loss of sktl activity in the notum since sktl mutant clones fail to grow in wing imaginal discs (C.P., unpublished results).
For example, Drosophila E-cadherin, an essential component of adherens junctions, is necessary for epithelial maintenance [22], and mutant (hypomorph) clones fail to form adherens junctions and lose their epithelial integrity completely.
However, these cell clones fail to compete with wild type cells and subsequently undergo apoptosis (supplementary material Fig. S6).
In Drosophila, IP3 receptor germline clones fail to produce viable embryos and ovary extracts detect expression of the IP3 receptor (Acharya et al., 1997; Chintapalli et al., 2007; McQuilton et al., 2012).
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