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In these knockdown (KD) clones, expression of the 2 HMTs was reduced by 80 90% (Figs. 1A and 1B).
In the rdx11 homozygous clones, expression of decapentaplegic (dpp) and patched (ptc), both of which are Ci target genes [1], [2], was not up-regulated in 2 4 cells anterior to the A/P boundary (Figure S2A, B), although dpp expression levels were enhanced in the more anterior region, where Rdx is expressed at moderate levels (Figure S2A-b, arrow).
For all clones, expression of d2eGFP was induced with statistical significance by the presence of alfacalcidol relative to the no drug control.
In all of the PNT1a-clu clones, expression of exogenous clusterin was significantly higher compared to the endogenous clusterin levels in the controls (PNT1a-hyg), but differences between the single clusterin clones were observed.
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To understand the molecular mechanism of drug resistance in the clones, expressions of drug resistance-related genes were examined.
In screening of libraries derived by expression cloning, expression of active proteins in E. coli can be limited by formation of inclusion bodies.
Further, for immunocytochemical localization of virus proteins (NS3), and cloning expression of NS3 genes [ 75] allow to detect neurovirulence generated by nonstructural JE virus proteins [ 75].
Therefore, various systems have been developed to enable efficient large-scale cloning and expression of ORF clones.
Test candidate clones for expression of the targeting transgene (mCherry in our example) to eliminate clones with secondary rearrangements.
Reverse transcriptase PCR (RT-PCR) analysis of these clones confirmed expression of hESC-specific mRNAs (Fig. 5).
Combination of cul3 mutant clones with expression of UAS-kel resulted in further increased dendritic arbor complexity (Table 2).
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