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For each cloned transductant, four separate clones expressing a 1 1 ratio of endogenous to transduced transcript were independently evaluated for Ank protein expression.
Function-driven analysis seeks to identify clones expressing a desired trait or useful activity, followed by biochemical characterization and sequence analysis.
Clones expressing a similar amount of ENPP1 were selected (Figure S1 C).
Stably transfected clones, expressing a fluorescent reporter, were obtained as previously described [ 81].
MARCM clones expressing a fusion protein between the DNA-binding domain of Sd and Ncoa6 (SdDB-Ncoa6) resulted in rounded clone morphology and dramatically increased Diap1 levels.
We investigated this possibility, by generating stable MM cell line-derived clones expressing a cyclin D1-green fluorescent protein (GFP) fusion protein (D1-GFP) or GFP alone.
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As shown in Fig. 4c, all cell clones expressing the verified shRNAs showed a significant reduction of p24 in the culture supernatant in comparison to cell clones expressing an HIV-1 irrelevant shRNA (scr).
We individually tested 2500 clones expressing an acrB variant and observed that 1.1% of the clones had better survival in presence of 1-hexene relative to the strain encoding the wild type pump.
Moreover, the clones express a higher amounts of stemness marker OCT4 and tendon specific transcription factor Scleraxis (SCX) mRNA, but a lower level of decorin (DCN).
Consistent with this, β-DG immunoreactivity was undetectable in the knockout clones, while the wildtype clones expressed a 43 kDa protein on Western blot (Figure 1C) and immunocytochemistry (Figures 1D, E).
These T cell clones expressed a Vα6 TCR, distinct from the Vα4.11 TCR expressed by the CT T cell clone (Fig. 6a).
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