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In all clones except the high expressor 27Flip/Cre15, a steady decline in recombination activity was evident as OHT exposure time decreased: for 27Flip/Cre15 flipping was already at ∼100% in the untreated population.
In the secondary screen, Tn Rv0431 induced higher levels of fluorescence in all IR clones except the RANTES_AmCyan IR line (Figure 5B), the IR clone that gave the fewest verifiable results.
The final set of parameters we obtained was able to reproduce accurately the experimental data for all clones except the unique bi-modal one, C7, for which the simulated distribution fit only the high modality.
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The cloned inserts were further confirmed by Southern blot analyses using the original BAC clones as probes; the results indicated that the digest patterns of the BGM recombinants were identical to those of the original BAC clones, except for the fragments derived from the ends of the insert.
All cDNA clones (excepting random non-fixed mutations) from A. absinthium A. tridentata and M. matricarioides contained the same (5'-GGCTTGGGTG-3') variant of the C-box (termed C*-box) whereas the other studied species displayed the canonical 5'-AGGATGGGTG-3'motif [ 31].
All the clones except for the control genes such as GAPDH, β-actin, tubulin and an EST highly similar to GAPDH, are duplicated on the array.
Our results, obtained by transferring the mutant mtDNA from heteroplasmic fibroblasts into cybrids, after selecting mtDNA isogenic clones except for the m.3890G>A/ MT- ND1 mutant position, establish the pathogenic role of this rare mutation in affecting CI function.
All PCR-derived DNA fragments were sequenced after cloning, except the RFP-expression cassette in pIVD144-eqFP611-IVD145-smGFP4.
Mean shoot length of Kawa was greater than for San Rosa, Fraser, Veronese, and PN471, and it had greater shoot mass than all other clones, except for the mass of Geyles, which did not vary significantly from that of Kawa.
The protocol described above was used to show binding of individual scFvs fused to phage particle clones, except that the supernatant fluids from centifuged microtitre plates were diluted 1 1 with PBS containing 0.2% Tween 20 and 4% MP.
The digestion patterns of iREX/BAC1 were identical to those of the original BAC clone, except for the fragments derived from the ends of the insert.
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