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Clones displaying a CV above 5% between a minimum of 3 replica spots were discarded from further analysis.
This screening method works by positive clones displaying a trait (as the result of the expression of a library gene) which is directly observable.
The authors say that clones displaying a 1.3-fold improvement are generally verified as authentically displaying an increase in activity upon repeat examination.
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Of thirty three T-cell clones displaying an IDO-specific lytic capacity, four clones were selected for further expansion due to a superior growth rate.
Under these conditions, the migratory behavior of the control and COUP cells was dramatically altered, with both clones displaying an approximately similar migration potential.
OC precursors were treated with CM derived from control or MDA-MB-231 cells transfected with scrambled shMMP-13 or with CM from one of the clones displaying an almost complete protein silencing (clone #1).
These clones displayed a CD4+CD8dim+ (TC5) and CD4+ CD8– (TC7) phenotype and mediated a specific major histocompatibility complex class I-restricted cytotoxic activity toward Cou-LB autologous tumor cell line.
Both clones display a mixed serotonergic and dopaminergic phenotype (Baudry et al. 2015).
These clones displayed a clear tendency to expand within the boundary along the mediolateral (ML) axis (see white arrows in flat mounted-hindbrains of Figure 1B); indeed, all clones that respected boundaries displayed a larger ML than AP length.
Based on restriction analysis with several four base cutters, the pENTR dTOPO clones derived from each species could be classified into 4 classes whereas the pBINPLUS clones displayed a single digestion pattern.
Furthermore, while the clones expressing the wild type hFXN presented normal fibroblast morphology (Fig. 3, line hFXN-1D12), the hFXNG130V expressing clones displayed a slightly altered morphology with smaller or less spread out cells (Fig. 3, line hFXNG130V-G4).
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