Four out of 102 cell clones displayed the desirable target, approximately 3.9%.
All cells within individual clones displayed the same T cell receptor V-beta family usage confirming clonality (data not shown).
While there is no PO-BW polymorphism in the amplified region, both embryonic and placental bw×po clones displayed the expected 50∶50 methylated (maternal): unmethylated (paternal) ratio (Fig. 2).
As described in vitro, we observed that both HIF2α clones and both HIF2α(1 485) clones displayed the same behaviour, respectively.
The clones displaying the same expression levels between TPD and healthy trees were effectively excluded by reverse Northern blot analysis.
Both clones displayed the same TCR beta amino acid sequence identical to the CMV-specific sequences previously reported in other patients (V beta 7-6, J beta 1-4, CASSLAPGATNEKLFFNEKLFF) (Price et al, 2005 ; Venturi et al, 2008 ).
As expected, all the wild-type neuroblast clones displayed the neuroanatomy of the central complex lineage as described above (ventrally position cell bodies, axon projection via the loVM and LEp tracts and innervations in the lateral accessory lobe and central complex. Figure 3B E,J M).
When possible, it is therefore advisable to develop a growth-based screening method because the method is simple (each library can be plated on a limited number of plates, as very few clones will grow) and enables high-throughput (only the clones displaying the desired activity are able to grow).
A further FISH analysis, using the subtelomeric 6p and 6q BAC/PAC clones, displayed the absence of both signals proving that the formation of the ring results from terminal deletions involving both the long and the short arms of chromosome 6 with a following fusion of the ends of the distal segments.
Based on the phage capture assay, the phage clone displaying the fusion peptide ATRSANGM showed highest affinity and specificity against t-PSA.
