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To our surprise, PTEN clones display increased levels of apoptosis, and selective inhibition of apoptosis within the clones results in tremendous hyperplastic overgrowth.
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We then promoted the selection of clones displaying increased binding affinity by allowing the mutants to evolve through sequential cycles of equilibrium-based selection using decreasing concentrations of ELR+ CXC chemokines.
PRDM16 transgenic mice display increased energy expenditure.
Interestingly, MCF-7-14 and its invasive clone CL6 cells displayed increased CD44 expression and downregulated CD24 expression compared with MCF-7 cells.
In addition, MCF-7-14 and its invasive clone CL6 cells displayed increased expression of CD44 and downregulated expression of CD24 compared with MCF-7 cells.
Similar to Khalili et al (2012), who found that introducing BRAFV600E into melanocytes increased expression of IL-1 β, clones 7 and 21 displayed increased IL-1 β mRNA and this expression was antagonised by PLX4032.
As previously reported, posterior midgut Apc1 −/− mutant clones contained significantly more cells and displayed increased ISC proliferation when compared with control (MARCM Lac-Z) clones (Cordero et al, 2012a; Fig 4A, B, E and F).
22Rv1 Snail high clone, which displayed the highest levels of Snail, also displayed increased migration (p = 0.06) and invasion (**p < 0.01) as compared to 22Rv1 Neo control.
Only five displayed increased expression.
Although under high-glucose growth conditions the lines showed little difference in viability, upon starvation or treatment with toxicants the NiPSCs from the two SNCA_Tri clones displayed significantly increased apoptosis, delayed protein import to organelles and reduced catabolism of aggregated proteins compared with the control lines.
Interestingly, in clone 2 with repeated passage the cells displayed increasing expression of C/EBPα and decreasing expression of AR and PSA.
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