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Similar to the FCHO2 TALEN lesions, the selected clones display an ApaI-resistant band(s) in addition to the two 387- and 150-bp cleavage products of the 537-bp PCR amplicon.
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Both clones display a mixed serotonergic and dopaminergic phenotype (Baudry et al. 2015).
Therefore, as both the G130V and I154F mutant clones display a stable and spontaneous biochemical phenotype and are able to proliferate in classical culture conditions, these new cellular models will be useful for functional-based large-scale drug screening.
BACs with large insert clones display a lower variation compared with the oligo platform.
Only 50% of follicles with somatic sax clones display a mild phenotype (compare supplementary material Fig. S1H with Fig. 3A,B).
In this vector, phage clones display a given protein fragment on the phage surface only when the insert's correct reading frame matches that of gpD.
These clones display a complete downregulation of endogenous XAF1 protein levels and abrogate XAF1 induction even after 24 hours of IFN-β treatment.
Thus, upon random integration, individual cell clones display a highly heterogenous expression pattern and have to be screened for appropriate expression.
In contrast, EcRDN clones display a medially displaced boundary, a phenotype that has been attributed to a delayed proneural wave (Reddy et al., 2010; Yasugi et al., 2008, 2010).
Of thirty three T-cell clones displaying an IDO-specific lytic capacity, four clones were selected for further expansion due to a superior growth rate.
It is of note that the antisense clones displayed an upregulated MIF-sense mRNA.
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