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Both clones display a mixed serotonergic and dopaminergic phenotype (Baudry et al. 2015).
Therefore, as both the G130V and I154F mutant clones display a stable and spontaneous biochemical phenotype and are able to proliferate in classical culture conditions, these new cellular models will be useful for functional-based large-scale drug screening.
BACs with large insert clones display a lower variation compared with the oligo platform.
These clones display a complete downregulation of endogenous XAF1 protein levels and abrogate XAF1 induction even after 24 hours of IFN-β treatment.
Only 50% of follicles with somatic sax clones display a mild phenotype (compare supplementary material Fig. S1H with Fig. 3A,B).
Thus, upon random integration, individual cell clones display a highly heterogenous expression pattern and have to be screened for appropriate expression.
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Similar to the FCHO2 TALEN lesions, the selected clones display an ApaI-resistant band(s) in addition to the two 387- and 150-bp cleavage products of the 537-bp PCR amplicon.
These clones displayed a CD4+CD8dim+ (TC5) and CD4+ CD8– (TC7) phenotype and mediated a specific major histocompatibility complex class I-restricted cytotoxic activity toward Cou-LB autologous tumor cell line.
These clones displayed a clear tendency to expand within the boundary along the mediolateral (ML) axis (see white arrows in flat mounted-hindbrains of Figure 1B); indeed, all clones that respected boundaries displayed a larger ML than AP length.
Based on restriction analysis with several four base cutters, the pENTR dTOPO clones derived from each species could be classified into 4 classes whereas the pBINPLUS clones displayed a single digestion pattern.
Furthermore, while the clones expressing the wild type hFXN presented normal fibroblast morphology (Fig. 3, line hFXN-1D12), the hFXNG130V expressing clones displayed a slightly altered morphology with smaller or less spread out cells (Fig. 3, line hFXNG130V-G4).
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