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The results reported here on control samples show a frequency of error spanning from 1/2000 (G6PD control clones) to 1/5000 (HPRT control clones), depending on the sequence composition, possibly because of the known nearest neighbour nucleotide effect on the accuracy of replication [27], [28].
On average 97.78% or more clones, depending on the library, were from a single chromosome arm.
A similar pattern of activation versus repression was exhibited by the remaining clones depending on their relative levels of ERα.
These data are consistent with previous observations indicating BIM expression can be induced upon TCR triggering in human CTL clones, depending on the agonistic peptide used [ 28, 29].
However, a concomitant increase in CAT or GPx in double transfectants corrected or overcorrected the hypersensitivity of the SOD clones depending on the ratios of activities CAT/SOD or GPx/SOD.
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There are exceptions: Napster and Uber clones depend on the other guy making some huge mistake.
However this necessitates extensive screening, because the chances of isolating high-producing clones depend on the number of clones that have been screened.
The behaviour of TkvQD-expressing clones depends on the position of these clones along the anterior posterior axes (Burke and Basler, 1996; Rogulja and Irvine, 2005).
In each case, the evolved clone quickly outcompeted the version that did not secrete invertase (Table 4), demonstrating that growth of all the evolved clones depends on secreted invertase.
We asked whether epigenetic drifts within populations are affected by the resulting positive or negative selection of the aged cells and how expansion of the aged clones depends on the DNA-methylation machinery.
Our results here have revealed that the accumulation of F-actin in RhoGEF2-expressing clones depends on Rho1 and Rok as expected; however, knocking down Limk, Dia or PKN did not significantly reduce F-actin levels in RhoGEF2 + Ras ACT-expressing clones.
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