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Results: Our restriction endonuclease and sequence analyses of 800 clones demonstrate that 75% of the clones carry an insert larger than 0.5 kb.
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Phylogenetic analysis of approximately 400 bacterial clones demonstrated that 96% were members of the Proteobacteria.
Biochemical characterization of some of the evolved TPI clones demonstrated that the Km for the TPS substrates was similar to that of the native TPS; however and in agreement with the very slow complementation phenotype, the kcat was 4 orders of magnitude lower, indicating that substrate binding played a major role on selection.
Sequencing of 20 clones demonstrated that the OAGR concatemer junction corresponded to the sequence of the oligo, effectively making a deletion that includes the ITR junction.
Sequence analysis of a number of obtained TAR clones demonstrated that most of the isolated var genes derived from the group which the cloning vectors had targeted, indicating that the selected targeting sequences occurred sufficiently specific to isolate var genes of a defined group.
Results from these five clones demonstrated that in the absence of HIF, the levels of DR5 mRNA were markedly decreased.
(J ) A comparison between multiple control and multiple loss of Notch function clones demonstrates that DCN patterns with clustered axons show a significant increased number of medulla axons.
Immunocytochemical analysis using stable ES clones demonstrated that FLAG-Nup98-HoxA9 had a similar localization pattern to transiently expressed EGFP-Nup98-HoxA9 in ES cells.
Gene expression profiling of early passage, presenescent TCCs compared to late passage, senescent clones demonstrated that 43 genes were differentially expressed in T-cell immunosenescence.
Sequence analyses of selected AS-specific clones demonstrated that about 50% contained open reading frames (ORFs) with codon usage unusual for G. tigrina [ 17].
DNA sequencing analysis of selected functional clones demonstrated that all of the functional clones were identical and that mutation of the linker sequence, in which Gly is changed to Cys and Ser, has changed to Pro (Table 1-wrap>).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com