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However, variation in nutrient use efficiency that exists among clones could affect growth responses.
For example, it is proposed that mutations in genes in the mesenchymal cell clones could affect their cellular proliferation, leading either to agenesis or reduction in size of a tooth.
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We next tested whether the orientation of sgRNA targeting and the location of target genome segments could affect the cloning efficiency.
It was revealed that number of colonies is significantly lower in case of HCT-116+/+ and DLD-1 cells transfected with miR-215-5p miR-215-5p miR-215-5pT-116−/− and HT-29 cells indicating that this mimicscould affect the cell clone formation butmechanotms that are at least in part dependent on p53 functionality.
This was done to address the concern of adjusting for local copy number changes that could affect estimation of the clone size with a gene alteration.
There are fears that the broad phrasing of the bill could affect the discussion of such topics as IVF or cloning.
Five clones, including SO3896, contained errors in the oligo-encoded 5' or 3' adapter att sequences that could affect the frequency of subsequently recombination into destination plasmids.
We verified by sequencing (the TOPO TA cloning kit; Invitrogen) that no sequence polymorphism was present in the primers that could affect the PCR efficiency.
A filtering system was required to remove those tags, which could affect interpretation of our clonality data and reduce the accuracy of the clone size measurement.
To investigate whether knockdown of WASF3 could affect metastasis of prostate cancer cells in vivo, as predicted from the invasion assays, we subjected selected clones to an experimental metastasis assay.
It could affect sales".
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