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The RNA was selected by hybridization to a series of cosmid clones containing the entire viral genome in partially overlapping segments.
Five independent clones containing the natural chicken ovomucoid gene have been isolated from a chicken gene library.
The resulting colony forming units are each comprised of bacterial clones containing the same plasmid reflecting a single allele in the original PCR reaction.
Moreover, we observed PBZ sensitivity across a panel of JFH-1 based inter-genotypic HCV clones containing the structural regions of diverse HCV genotypes (1 6) (Fig. 2I).
Notsu et al. (2002) used plasmid clones containing the "shotgun" DNA fragments derived from isolated mitochondrial DNA for sequence analysis employing a conventional chain terminating method.
The sequencing results indicated that the PCR polymerase had very high fidelity, as the 30 different positive clones containing the cueO gene did not contain any mutations.
To date we have identified a total of 17 clones containing the hypoxanthine-guanine phosphoribosyltransferase, human serum albumin-human alpha-fetoprotein, p53, cyclooxygenase I, human apurinic endonuclease, beta-polymerase, and DNA ligase I genes.
Clones containing the proper insert were identified with PCR.
Templates were then PCR screened to identify individual clones containing the gene of interest.
This pattern is typical of BAC clones containing the chloroplast genome [19].
Entry clones containing the Lifeact peptide and mEGFP were generated via BP clonase from PCR products.
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