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In using pooling designs to identify clones containing a specific subsequence called positive clones, sometimes there exist nonpositive clones which can cancel the effect of positive clones.
Twelve clones containing a correctly targeted allele were identified.
Anti-macrophage activity is also seen in response to positive clones containing a phospholipase D domain protein.
However, this strategy cannot be used to isolate clones containing a second intron insertion in an already erythromycin-resistant mutant.
Only fosmid clones containing a putative full carotenoid biosynthetic pathway leading to rhodopsin and a clear phylogenetic identity were included in the dataset to best facilitate pathway reconstruction.
In parallel, mock-transfected cell clones were also developed bearing empty vector only, as well as mutant clones containing a putative PTPase-dead mutant Tensin3 (Tns3 Mut) cDNA.
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BACs are circular F-element-based circular bacterial genomic clones containing an average of about 180 200 kb genomic DNA.
The vector-harboring clones containing an insert of appropriate sizes (about 1500 bp) were cultured on Luria-Bertani (LB) plates by standard methods (Kaku et al. 2005).
In marked contrast, most ES cell clones containing an integrated codon-optimized ΦC31o construct displayed robust X-gal staining.
The fraction of clones containing an insert is then investigated by colony PCR on the obtained transformants.
One of 36 clones contained a cDNA insert with a near full-length human COMMD1 coding sequence (amino acids 5 190).
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