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Therefore, breeding effects on physiology (e.g. water use efficiency) should be evaluated in order to quantify the global differences among commercial seed lots or clones compared with unimproved material.
Flow cytometric measurements revealed an increase in Δψm in both cybrid clones compared with ρ0 cells.
Blast2 alignment results from PCR clones compared with the wild-type reference sequence were first saved as text files.
G9a KD cells showed increased DNA content (1.7-fold in 2 independent clones) compared with FACS analyses to control.
We observed a lack of significant decrease of CD4 surface expression in shRNA clones compared with wild-type Jurkat cells or shSCRAM clone.
Analysis of protein levels by Western blotting confirmed reduced levels of protein expression in #1029 clones compared with controls (Figure 1B).
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No significant difference in growth rate of S. cerv-SK clone, compared with the controls, was observed).
The quantity of recombinant protein was the highest in the TtsfGFP clone compared with sfGFP and EGFP.
In our screen, a more than two-fold decreased amount of EGFR occurred, collected from A549/CCN2 clone, compared with control clone (Supplementary Figure S1).
Despite the substantial genetic diversity of this clone compared with other fully sequenced S. aureus, very few clone-specific coding sequences were identified.
We used the opportunistic pathogen Pseudomonas aeruginosa to test the hypothesis that siderophore cheats will have a selective advantage in mixed-clone compared with single-clone infections, and that their presence will reduce virulence.
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