Sentence examples for clones bound to from inspiring English sources

After three rounds of subtractive panning, a majority of clones bound to DU145 cells as detected by flow cytometry.

Two clones bound to the HMGB1 box A, the other two could only bind to the box A+B, failing to recognize the box A or box B (Fig. 2B).

From these 4 binders, one was specific for an epitope only present on P. pastoris-derived ΔNΔC-VEGF-C, which was used for selection, while the other 3 clones bound to an epitope in the conserved region of ΔNΔC-VEGF-C.

None of the novel clones bound to any of these substrates demonstrating that polyreactive binding properties had not developed as a consequence of the affinity maturation process.

In this study, we have incubated the phage-displayed scFv library with PLAP and eluted the phage clones bound to the immobilized PLAP by L-Phe-Gly-Gly in the presence of pNPP.

To further confirm that these PAB clones bind to human PSP94 protein, we used another ELISA sandwich layout in which in vivo biotinylated PAB clones were immobilized to a coated streptavidin and binding of His-PSP94 was detected by anti-PSP94 antibody followed by a secondary IgG-HRP conjugate (results not shown).

In a competition assay, 10C3 and 4E10 did not compete with 2A12, while 4E10 partially competed with 10C3, suggesting that these three clones bind to different epitopes on MIF (Fig. 1B).

In concordance with the cell-based ELISA findings (Figure 5, C), the 6_2G3 clone bound to a range of melanoma cell lines, but not to melanocytes (Figure 6).

One antibody clone bound to mouse TNF-alpha with a high affinity (Kd = 1.4 nM, measured by Surface Plasmon Resonance on a Biacore3000, Fig. 3A), and good specificity (the epitope mapped to amino acids 19 40 of the TNF-alpha mature chain, data not shown), and could neutralize mouse TNF-alpha activity by blocking TNF-alpha's cytotoxic effect on L929 cells (Fig. 3B).

A general observation from the experiments performed is that the Rps8, Wfdc17, Cbx3, Rnf130, and Sec61b clones often bound to sera better than other clones such as Mxd1 and the poly T clones.

Among these, eighteen (2nd round) and nineteen (3rd round) phage clones specifically bound to the mAb VT80.12.