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However, it was possible to use PfuUltra II Fusion to amplify the plasmid clones because the primers hybridized perfectly to the plasmid template.
Our model is not intended to distinguish among multiple clones because the state and proportion of tumor component cannot always be uniquely determined from the genotyping data of the mixed sample.
We are unable to generate loss-of-function mosaic clones because the Dnhe2 genomic locus is too close to the nearest flippase recognition site (FRT40A).
In the remaining 43 cases, we were unable to match peaks to clones because the clone was rare, resulting in low confidence of assignment.
This difference in source distribution was further demonstrated for individual clones because the human/food ratio of both CC1 (2.6) and CC2 (2.8) differed significantly from those of CC3 (0.65) and CC9 (0.5) (χ p<0.01 for the 4 comparisons).
This also explains the greater gene discovery potential of SalI MSLL clones, because the recognizable coding exons of genes are usually several hundred bp to a few kb away from the epigenetic boundary where SalI cuts.
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The difficulty in distinguishing between damage-induced false clones and real clones arises because the clones are negatively marked by the absence of GFP signal.
(We're going to ignore the fact that the first stormtroopers were all clones, because a) the clone troopers were phased out during the Empire's post-Clone Wars consolidation, and b) the prequels were terrible).
It's not cloning because the egg would be fertilized with a sperm cell, but cloning research established the viability of the technique.
Scientists use the term "somatic cell nuclear transfer" (SCNT) when they talk about cloning because the phrase is both technically correct and a lot less controversial.
These overhangs are very useful in cloning because the unpaired nucleotides will pair with other overhangs made using the same restriction enzyme.
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